Kev tshuaj xyuas online ua kom ntseeg tau tias cov ntsiab lus ntawm cov substrate yog qis dua qhov pib, ua kom muaj kev ua haujlwm ntawm cov enzymes lom neeg thoob plaws hauv cov txheej txheem, thiab ua kom cov tshuaj tiv thaiv hydrolysis ntau dua.
Amide tebchaw yog ib qho tseem ceeb ntawm cov organic synthesis intermediates thiab tshuaj thiab yog dav siv nyob rau hauv tshuaj, tshuaj tua kab, zaub mov, kev tiv thaiv ib puag ncig, roj ntau lawm thiab lwm yam teb.Cov tshuaj tiv thaiv hydrolysis ntawm cov pab pawg nitrile mus rau hauv pawg amide yog ib txoj hauv kev tseem ceeb rau kev npaj amide compounds hauv kev lag luam.
Lub biocatalyst yog siv nyob rau hauv cov txheej txheem ntsuab synthesis ntawm ib tug tej yam amide compound, thiab nws cov kev ua yog heev cuam tshuam los ntawm cov concentration ntawm substrate thiab cov khoom nyob rau hauv lub system.Yog hais tias lub substrate concentration yog siab dhau lawm, lub catalyst yuav yooj yim deactivated, ua rau nws tsis yooj yim sua mus txuas ntxiv cov tshuaj synthesis;Yog hais tias cov khoom concentration siab dhau lawm, nws tseem yuav ua rau tsub zuj zuj ntawm substrate thiab tsis tshua muaj synthesis efficiency.Txhawm rau kom ntseeg tau tias qhov kev ua haujlwm zoo ntawm cov enzyme catalysts hauv kev sib xyaw ua ke, cov cuab yeej siv tau zoo yuav tsum tau saib xyuas thiab tawm tswv yim kho cov concentrations ntawm nitrile substrates thiab amide cov khoom hauv lub sijhawm tiag tiag thaum lub sijhawm cov txheej txheem tshuaj tiv thaiv.
Tam sim no, txoj hauv kev xws li kev ua piv txwv ntawm lub sijhawm tas mus li thiab ua cov roj chromatography-mass spectrometry tom qab kuaj ua ntej kev kho mob feem ntau yog siv los txheeb xyuas cov substrate thiab cov ntsiab lus ntawm cov khoom hauv cov tshuaj tiv thaiv.Kev tshawb nrhiav offline tau lag luam, cov xwm txheej tam sim no tsis tuaj yeem paub txog lub sijhawm tiag tiag, thiab nws nyuaj rau kev tswj xyuas cov lus pom zoo thiab kev hloov pauv ntawm cov ntsiab lus substrate, thiab lub sijhawm pub mis zoo tshaj plaws yuav ploj mus.Online spectral tsom xam tshuab muaj qhov zoo ntawm kev tshawb nrhiav ceev ceev thiab tsis xav tau cov qauv ua ntej.Nws muaj peev xwm paub ceev ceev, real-time, nyob rau hauv-situ thiab ntse tsom xam ntawm cov tshuaj tiv thaiv kab mob, thiab muaj exceptional zoo nyob rau hauv lub ntsuab synthesis ntawm amide tebchaw.
Daim duab saum toj no qhia txog kev saib xyuas online ntawm cov txheej txheem ntawm kev npaj acrylamide los ntawm bioenzymatic cov tshuaj tiv thaiv ntawm ib co nitrile compound.Los ntawm 0 mus rau t1 tom qab cov tshuaj tiv thaiv pib, tus nqi noj ntawm nitrile raw cov ntaub ntawv kuj loj, thiab cov tsub zuj zuj ntawm cov substrate thiab cov khoom yog ceev ceev.Ntawm t1, cov ntsiab lus ntawm cov substrate yog ze rau qhov siab tshaj ntawm qhov pib.Lub sijhawm no, cov neeg ua haujlwm tsim khoom txo qis cov khoom noj ntawm cov khoom siv raw kom cov substrate concentration nyob rau hauv cov tshuaj tiv thaiv kab mob nyob rau hauv ib tug controllable ntau yam, thiab cov khoom tseem tuaj yeem khaws tau sai.Thaum kawg, thaum cov tshuaj tiv thaiv mus rau lub sij hawm t2, cov khoom cov ntsiab lus accumulates mus rau lub hom phiaj, thiab cov neeg ua hauj lwm ntau lawm nres ntxiv nitrile raw khoom.Tom qab ntawd, theem substrate mus zero thiab cov ntsiab lus ntawm cov khoom kuj zoo li ruaj khov.Thaum lub sij hawm tag nrho cov txheej txheem tas mus li, kev soj ntsuam hauv online kom ntseeg tau tias cov tshuaj lom neeg enzyme catalytic ua tau zoo.
Hauv kev sib xyaw ua ke loj, kev soj ntsuam online thev naus laus zis tseem ceeb tshwj xeeb.Kev paub txog lub sijhawm tiag tiag ntawm substrate thiab cov khoom ntim khoom tuaj yeem pab cov tswv yim los kho cov ntsiab lus substrate hauv qhov tsim nyog.Thaum lub sij hawm cov txheej txheem cov tshuaj tiv thaiv, nws muaj peev xwm ua kom cov kev ua si ntawm cov roj ntsha enzyme catalyst, txhim kho cov efficiency ntawm synthesis cov tshuaj tiv thaiv, thiab pab tswj cov txheej txheem tsis nyob rau hauv lub zoo lub xeev.Nthuav lub neej kev pab cuam ntawm cov roj ntsha enzyme catalysts thiab ua kom cov txiaj ntsig zoo tshaj plaws.
Post lub sij hawm: Jan-23-2024